Noninvasive, nondestructive approaches to cell bioenergetics.

Non-invasive approaches to tissue bioenergetics.

It is clear that continuous light affords a very limited window of opportunity for quantitative spectrophotometry of brain tissue. However, the number of qualitative applications which are available exemplify how this extremely simple technique can be applied to important medical problems. The development of devices which can measure directly the oxygen saturation of the brain is more complicat...

Uncoupling: new approaches to an old problem of bioenergetics.

Review of methods to probe single cell metabolism and bioenergetics.

Single cell investigations have enabled unexpected discoveries, such as the existence of biological noise and phenotypic switching in infection, metabolism and treatment. Herein, we review methods that enable such single cell investigations specific to metabolism and bioenergetics. Firstly, we discuss how to isolate and immobilize individuals from a cell suspension, including both permanent and...

Foxg1 localizes to mitochondria and coordinates cell differentiation and bioenergetics.

Forkhead box g1 (Foxg1) is a nuclear-cytosolic transcription factor essential for the forebrain development and involved in neurodevelopmental and cancer pathologies. Despite the importance of this protein, little is known about the modalities by which it exerts such a large number of cellular functions. Here we show that a fraction of Foxg1 is localized within the mitochondria in cell lines, p...

Circadian Mechanisms in Bioenergetics and Cell Metabolism

Circadian clocks are biologic oscillators present in all photosensitive species that produce 24-h cycles in the transcription of rate-limiting metabolic enzymes in anticipation of the light–dark cycle. In mammals, the clock drives energetic cycles to maintain physiologic constancy during the daily switch in behavioral (sleep/wake) and nutritional (fasting/feeding) states. A molecular connection...